Paper , Order, or Assignment Requirements

Answer all three questions below, which relate to aspects of the microscopy used in the paper.

 

Percentages given in parenthesis indicate the relative value of each question.

 

Maximum word limits are specified for each question; please adhere to these limits.

 

Question 1

 

(format your answer as a table)

 

(i) What types of microscopy were utilised in the study?

 

(ii) For each type of microscopy:

 

1. a) Briefly describe the basic principle of how this microscopy works, and the key elements of specimen preparation.

 

1. b) What are its advantages and disadvantages?

 

1. c) Why was it used in this study?

 

Question 2

 

For immunofluorescence labelling, cells were incubated in monoclonal antibodies (JIM5, JIM7), followed by fluorescein-‐ and/or rhodamine-‐conjugated goat antirat antibodies.

 

(i) Explain the mechanism behind this procedure.

 

(ii) What is the difference between monoclonal and polyclonal antibodies, and how are they made. Why do you think they used monoclonal antibodies in this study?

 

(iii) How could additional immunolabelling have added to the precision of epitope localization, and which could have been portrayed in Figure 2?

 

Question

 

A number of photos depict cells that have been “colabelled”.

 

(i) What is colabelling, and what are its advantages over single labelling?

 

(ii) What combinations of stains have been used in this paper, and what cell components do they differentiate? (Cite a figure as an example of each combination).

 

(iii) How else might some of these images have been presented to even more clearly demonstrate the result of the colabelling