glucose-6-phosphate isomerase [Bacillus] protein Academic Essay

write a two-page research proposal.
Use BLAST (http://www.ncbi.nlm.nih.gov/ ) to identify the name and function of this protein and provide experimental details about molecular cloning (clone the gene of interest into an appropriate expression vector), the expression system, and purification technique. List potential challenges and discuss strategies for how to overcome them. I attached some paper for help

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